Regeneration of Full-Thickness Rotator Cuff Tendon Tear After Ultrasound-Guided Injection With Umbilical Cord Blood-Derived Mesenchymal StemCells in a Rabbit Model
Tissue Engineering and Regenerative Medicine
STEM CELLS TRANSLATIONALMEDICINE 2015;4:1344–1351 http://dx.doi.org/10.5966/sctm.2015-0040
GI -YOUNG PARK ,a DONG RAK KWON ,a,* SANG CHUL LEEb,*
Rotator cuff tendon tear is one of the most common causes of chronic shoulder pain and disability. In this study, we investigated the therapeutic effects of ultrasound-guided human umbilical cord blood(UCB)-derived mesenchymal stem cell (MSC) injection to regenerate a full-thickness subscapularistendontearinarabbitmodelbyevaluatingthegrossmorphologyandhistologyoftheinjectedtendonand motion analysis of the rabbit’s activity. At 4 weeks after ultrasound-guided UCB-derived MSCinjection, 7 of the 10 full-thickness subscapularis tendon tears were only partial-thickness tears,and3remainedfull-thicknesstendontears. Thetendon tearsizeandwalkingcapacityat4 weeksafterUCB-derived MSC injection under ultrasound guidance were significantly improved compared withthe same parameters immediately after tendon tear. UCB-derived MSC injection under ultrasoundguidance without surgical repair or bioscaffold resulted in the partial healing of full-thickness rotatorcuff tendon tears in a rabbit model. Histology revealed that UCB-derived MSCs induced regenerationof rotator cuff tendon tear and that the regenerated tissue was predominantly composed of type Icollagens. In this study, ultrasound-guided injection of human UCB-derived MSCs contributed to re-generation of the full-thickness rotator cuff tendon tear without surgical repair. The results demon-strate the effectiveness of local injection of MSCs into the rotator cuff tendon.
UCB-derived MSC injection under ultrasound guidance withoutsurgical repair or bioscaffold resulted in the partial healing offull-thickness rotator cuff tendon tears in a rabbit model. Histol-ogy revealed that UCB-derived MSCs induced regeneration of ro-tator cuff tendon tears and that the regenerated tissue waspredominantly composed of type I collagens. In addition, motionanalysis showed better walking capacity after MSC injectionthan HA or normal saline injection. These results suggest thatultrasound-guided UCB-derived MSC injection may be a usefulconservative treatment for full-thickness rotator cuff tendon tearrepair.