Adipose-derived mesenchymal stem cells markedly attenuate brain infarct size and improve neurological function in rats
Journal of Translational Medicine
Leu et al. Journal of Translational Medicine 2010, 8:63 http://www.translational-medicine.com/content/8/1/63
Steve Leu†1, Yu-Chun Lin1, Chun-Man Yuen†2, Chia-Hung Yen3, Ying-Hsien Kao4, Cheuk-Kwan Sun*†5 and Hon- Kan Yip*1,6
Background: The therapeutic effect of adipose-derived mesenchymal stem cells (ADMSCs) on brain infarction area (BIA) and neurological status in a rat model of acute ischemic stroke (IS) was investigated.
Methods: Adult male Sprague-Dawley (SD) rats (n = 30) were divided into IS plus intra-venous 1 mL saline (at 0, 12 and 24 h after IS induction) (control group) and IS plus intra-venous ADMSCs (2.0 × 106) (treated interval as controls) (treatment group) after occlusion of distal left internal carotid artery. The rats were sacrificed and brain tissues were harvested on day 21 after the procedure.
Results: The results showed that BIA was larger in control group than in treatment group (p < 0.001). The sensorimotor functional test (Corner test) identified a higher frequency of turning movement to left in control group than in treatment group (p < 0.05). mRNA expressions of Bax, caspase 3, interleukin (IL)-18, toll-like receptor-4 and plasminogen activator inhibitor-1 were higher, whereas Bcl-2 and IL-8/Gro were lower in control group than in treatment group (all p < 0.05). Western blot demonstrated a lower CXCR4 and stromal-cell derived factor-1 (SDF-1) in control group than in treatment group (all p < 0.01). Immunohistofluorescent staining showed lower expressions of CXCR4, SDF-1, von Willebran factor and doublecortin, whereas the number of apoptotic nuclei on TUNEL assay was higher in control group than in treatment group (all p < 0.001). Immunohistochemical staining showed that cellular proliferation and number of small vessels were lower but glial fibrillary acid protein was higher in control group than in treatment group (all p < 0.01).
Conclusions: ADMSC therapy significantly limited BIA and improved sensorimotor dysfunction after acute IS.
ADMSC therapy limited brain infarct size and improved neurological function in rats after acute IS through enhancement of angiogenesis/vasculogenesis and neurogenesis as well as its anti-inflammatory and anti-apoptotic effects.